Methods used to determine the constituents of the fibrous fraction, a review

The estimation of the chemical and bromatological composition of foods involves a series of studies that evaluate mainly the fiber fraction, since it presents great variability when compared to the other components. The growing number of publications on the analytical techniques available for determining the nutritional value of foods end up generalizing the use of ill-defined terms. There are several techniques for evaluating forage components, the detergent system being the most widely used, although there are more modern methods. However, the accessibility and cost of these modern methods are factors that limit their use in many laboratories. Furthermore, some of these methods are not recognized as official methods of analysis. In this context, the objectives of this review were: to highlight the most important concepts in the determination of the nutritional value of foods; to improve the use and the difficulties of interpretation of the analytical results. It was concluded that the use of analytical methods allows the estimation of the composition and the availability of the different fractions of the cell wall. But variability of the cell wall constituents requires knowledge of the different analytical methodologies available. The analytical methods, traditional or alternative, are still empirical since they present different results for the same analysis. These variations are generated most of the time by the differences between the steps of the analytical procedures. It is clear that the improvement of analytical methods is of paramount importance to estimate the nutritional value of foods

MUMAL: multivariate analysis in shotgun proteomics using machine learning techniques.

BACKGROUND: The shotgun strategy (liquid chromatography coupled with tandem mass spectrometry) is widely applied for identification of proteins in complex mixtures. This method gives rise to thousands of spectra in a single run, which are interpreted by computational tools. Such tools normally use a protein database from which peptide sequences are extracted for matching with experimentally derived mass spectral data. After the database search, the correctness of obtained peptide-spectrum matches (PSMs) needs to be evaluated also by algorithms, as a manual curation of these huge datasets would be impractical. The target-decoy database strategy is largely used to perform spectrum evaluation. Nonetheless, this method has been applied without considering sensitivity, i.e., only error estimation is taken into account. A recently proposed method termed MUDE treats the target-decoy analysis as an optimization problem, where sensitivity is maximized. This method demonstrates a significant increase in the retrieved number of PSMs for a fixed error rate. However, the MUDE model is constructed in such a way that linear decision boundaries are established to separate correct from incorrect PSMs. Besides, the described heuristic for solving the optimization problem has to be executed many times to achieve a significant augmentation in sensitivity. RESULTS: Here, we propose a new method, termed MUMAL, for PSM assessment that is based on machine learning techniques. Our method can establish nonlinear decision boundaries, leading to a higher chance to retrieve more true positives. Furthermore, we need few iterations to achieve high sensitivities, strikingly shortening the running time of the whole process. Experiments show that our method achieves a considerably higher number of PSMs compared with standard tools such as MUDE, PeptideProphet, and typical target-decoy approaches. CONCLUSION: Our approach not only enhances the computational performance, and thus the turn around time of MS-based experiments in proteomics, but also improves the information content with benefits of a higher proteome coverage. This improvement, for instance, increases the chance to identify important drug targets or biomarkers for drug development or molecular diagnostics

Estudo da suscetibilidade ao escurecimento por radiação gama de quartzo róseo-leitoso da província pegmatítica da Borborema

Este estudo considera as transformações induzidas pela radiação γ sobre os defeitos estruturais em quartzo róseo e leitoso da Província Pegmatítica da Borborema (Paraíba e Rio Grande do Norte, Brasil). Amostras de coloração rósea e leitosa foram irradiadas com doses de raios γ (60Co) entre 0,5 e 96 kGy. Defeitos pontuais relacionados às impurezas de Al, Ge, Li e grupos OH foram caracterizados por espectroscopias ultravioleta visível e infravermelha e ressonância paramagnética eletrônica, antes e após a irradiação. A espectrometria de massa com plasma indutivamente acoplado foi empregada para quantificar as concentrações de Al, Li, Ge, Fe, Ti e outras impurezas em fragmentos de diferentes tonalidades. Constatou-se que os defeitos [AlO4]0, [AlO4/H]0 e [GeO4/Li]0 são formados pela dissociação dos defeitos [AlO4/Li]0 e [Li-OH], a partir de doses de 0,5 kGy. A diminuição do sinal da ressonância paramagnética eletrônica do centro [GeO4/Li]0 e a formação de centros E'1 perturbados por Ge para doses acima de 8 kGy confirmou a grande mobilidade dos íons Li+ pela estrutura do quartzo. O aumento da população de defeitos [AlO4]0 e o consequente escurecimento das amostras foi semelhante para ambos os tipos de quartzo. Análises preliminares por microscopia eletrônica mostraram a presença de fibras nanométricas apenas no resíduo da digestão ácida de amostras róseas, indicando que a coloração rósea do quartzo da Província Pegmatítica da Borborema está provavelmente relacionada às inclusões de dumortierita

Autonomic and Renal Alterations in the Offspring of Sleep-Restricted Mothers During Late Pregnancy

OBJECTIVES: Considering that changes in the maternal environment may result in changes in progeny, the aim of this study was to investigate the influence of sleep restriction during the last week of pregnancy on renal function and autonomic responses in male descendants at an adult age. METHODS: After confirmation of pregnancy, female Wistar rats were randomly assigned to either a control or a sleep restriction group. The sleep-restricted rats were subjected to sleep restriction using the multiple platforms method for over 20 hours per day between the 14th and 20th day of pregnancy. After delivery, the litters were limited to 6 offspring that were designated as offspring from control and offspring from sleep-restricted mothers. Indirect measurements of systolic blood pressure (BPi), renal plasma flow, glomerular filtration rate, glomerular area and number of glomeruli per field were evaluated at three months of age. Direct measurements of cardiovascular function (heart rate and mean arterial pressure), cardiac sympathetic tone, cardiac parasympathetic tone, and baroreflex sensitivity were evaluated at four months of age. RESULTS: The sleep-restricted offspring presented increases in BPi, glomerular filtration rate and glomerular area compared with the control offspring. The sleep-restricted offspring also showed higher basal heart rate, increased mean arterial pressure, increased sympathetic cardiac tone, decreased parasympathetic cardiac tone and reduced baroreflex sensitivity. CONCLUSIONS: Our data suggest that reductions in sleep during the last week of pregnancy lead to alterations in cardiovascular autonomic regulation and renal morpho-functional changes in offspring, triggering increases in blood pressure

An Improved Description of the Dielectric Breakdown in Oxides Based on a Generalized Weibull distribution

In this work, we address modal parameter fluctuations in statistical distributions describing charge-to-breakdown $(Q_{BD})$ and/or time-to-breakdown $(t_{BD})$ during the dielectric breakdown regime of ultra-thin oxides, which are of high interest for the advancement of electronic technology. We reobtain a generalized Weibull distribution ($q$-Weibull), which properly describes $(t_{BD})$ data when oxide thickness fluctuations are present, in order to improve reliability assessment of ultra-thin oxides by time-to-breakdown $(t_{BD})$ extrapolation and area scaling. The incorporation of fluctuations allows a physical interpretation of the $q$-Weibull distribution in connection with the Tsallis statistics. In support to our results, we analyze $t_{BD}$ data of SiO$_2$-based MOS devices obtained experimentally and theoretically through a percolation model, demonstrating an advantageous description of the dielectric breakdown by the $q$-Weibull distribution.Comment: 5 pages, 3 figure

Organic film thickness influence on the bias stress instability in Sexithiophene Field Effect Transistors

In this paper, the dynamics of bias stress phenomenon in Sexithiophene (T6) Field Effect Transistors (FETs) has been investigated. T6 FETs have been fabricated by vacuum depositing films with thickness from 10 nm to 130 nm on Si/SiO2 substrates. After the T6 film structural analysis by X-Ray diffraction and the FET electrical investigation focused on carrier mobility evaluation, bias stress instability parameters have been estimated and discussed in the context of existing models. By increasing the film thickness, a clear correlation between the stress parameters and the structural properties of the organic layer has been highlighted. Conversely, the mobility values result almost thickness independent

Identificação molecular de Bartonella henselae em paciente com SIDA soronegativo para doença da arranhadura do gato no Rio de Janeiro, Brasil

Bartonella henselae is associated with a wide spectrum of clinical manifestations, including cat scratch disease, endocarditis and meningoencephalitis, in immunocompetent and immunocompromised patients. We report the first molecularly confirmed case of B. henselae infection in an AIDS patient in state of Rio de Janeiro, Brazil. Although DNA sequence of B. henselae has been detected by polymerase chain reaction in a lymph node biopsy, acute and convalescent sera were nonreactive.Bartonella henselae está associada a um amplo espectro de manifestações clínicas, incluindo a doença da arranhadura de gato, endocardite, e meningoencefalite, em pacientes imunocompetentes e imunocomprometidos. Relatamos o primeiro caso confirmado por método molecular de B. henselae em um paciente com SIDA no estado do Rio de Janeiro, Brasil. Apesar da sequência de DNA de B. henselae ser detectada pela reação em cadeia da polimerase em uma biópsia do linfonodo, soros das fases aguda e convalescente foram não reativos

The 1:1 resonance in Extrasolar Systems: Migration from planetary to satellite orbits

We present families of symmetric and asymmetric periodic orbits at the 1/1 resonance, for a planetary system consisting of a star and two small bodies, in comparison to the star, moving in the same plane under their mutual gravitational attraction. The stable 1/1 resonant periodic orbits belong to a family which has a planetary branch, with the two planets moving in nearly Keplerian orbits with non zero eccentricities and a satellite branch, where the gravitational interaction between the two planets dominates the attraction from the star and the two planets form a close binary which revolves around the star. The stability regions around periodic orbits along the family are studied. Next, we study the dynamical evolution in time of a planetary system with two planets which is initially trapped in a stable 1/1 resonant periodic motion, when a drag force is included in the system. We prove that if we start with a 1/1 resonant planetary system with large eccentricities, the system migrates, due to the drag force, {\it along the family of periodic orbits} and is finally trapped in a satellite orbit. This, in principle, provides a mechanism for the generation of a satellite system: we start with a planetary system and the final stage is a system where the two small bodies form a close binary whose center of mass revolves around the star.Comment: to appear in Cel.Mech.Dyn.Ast

On the dynamics of Extrasolar Planetary Systems under dissipation. Migration of planets

We study the dynamics of planetary systems with two planets moving in the same plane, when frictional forces act on the two planets, in addition to the gravitational forces. The model of the general three-body problem is used. Different laws of friction are considered. The topology of the phase space is essential in understanding the evolution of the system. The topology is determined by the families of stable and unstable periodic orbits, both symmetric and non symmetric. It is along the stable families, or close to them, that the planets migrate when dissipative forces act. At the critical points where the stability along the family changes, there is a bifurcation of a new family of stable periodic orbits and the migration process changes route and follows the new stable family up to large eccentricities or to a chaotic region. We consider both resonant and non resonant planetary systems. The 2/1, 3/1 and 3/2 resonances are studied. The migration to larger or smaller eccentricities depends on the particular law of friction. Also, in some cases the semimajor axes increase and in other cases they are stabilized. For particular laws of friction and for special values of the parameters of the frictional forces, it is possible to have partially stationary solutions, where the eccentricities and the semimajor axes are fixed.Comment: Accepted in Celestial Mechanics and Dynamical Astronom